Spore Cortex Hydrolysis Precedes Dipicolinic Acid Release during Clostridium difficile Spore Germination
نویسندگان
چکیده
منابع مشابه
Clostridium difficile Spore-Macrophage Interactions: Spore Survival
BACKGROUND Clostridium difficile is the main cause of nosocomial infections including antibiotic associated diarrhea, pseudomembranous colitis and toxic megacolon. During the course of Clostridium difficile infections (CDI), C. difficile undergoes sporulation and releases spores to the colonic environment. The elevated relapse rates of CDI suggest that C. difficile spores has a mechanism(s) to ...
متن کاملEffects of cortex peptidoglycan structure and cortex hydrolysis on the kinetics of Ca(2+)-dipicolinic acid release during Bacillus subtilis spore germination.
The kinetic parameters of the release of Ca(2+)-dipicolinic acid (CaDPA) during germination of spore populations and multiple individual spores of Bacillus subtilis strains with major alterations in the structure of the spore peptidoglycan (PG) cortex or lacking one or both of the two redundant enzymes involved in cortex hydrolysis (cortex-lytic enzymes [CLEs]) were determined. The lack of the ...
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Infections caused by Clostridium difficile have increased steadily over the past several years. While studies on C. difficile virulence and physiology have been hindered, in the past, by lack of genetic approaches and suitable animal models, newly developed technologies and animal models allow these processes to be studied in detail. One such advance is the generation of a mouse-model of C. dif...
متن کاملInhibiting the initiation of Clostridium difficile spore germination using analogs of chenodeoxycholic acid, a bile acid.
To cause disease, Clostridium difficile spores must germinate in the host gastrointestinal tract. Germination is initiated upon exposure to glycine and certain bile acids, e.g., taurocholate. Chenodeoxycholate, another bile acid, inhibits taurocholate-mediated germination. By applying Michaelis-Menten kinetic analysis to C. difficile spore germination, we found that chenodeoxycholate is a compe...
متن کاملRibonucleic acid synthesis during fungal spore germination.
Spores of several fungi were examined in an effort to understand the role of RNA in the biochemical events occurring early in germination. RNA synthesis appeared to be an essential requirement for germination in Neurospora crassa and Aspergillus nidulans but not in Alternaria solani and Peronospora tabacina. Gross changes in the relative amounts of the various molecular species of RNA were not ...
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ژورنال
عنوان ژورنال: Journal of Bacteriology
سال: 2015
ISSN: 0021-9193,1098-5530
DOI: 10.1128/jb.02575-14